| Author(s) | ABOULENEIN HY; ISLAM MR
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| Title | DIRECT HPLC SEPARATION OF THALIDOMIDE ENANTIOMERS USING CELLULOSE TRIS-4-METHYLPHENYL BENZOATE CHIRAL STATIONARY PHASE
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| Source | JOURNAL OF LIQUID CHROMATOGRAPHY 14(4):667-673
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| Date | 1991
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| Type | Journal : Article
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| LCR: 1 NCR: 6 LCS: 9 GCS: 17
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| Reprint | ABOULENEIN, HY
KING FAISAL SPECIALIST HOSP & RES CTR,DEPT RADIONUCLIDE & CYCLOTRON OPERAT,DRUG DEV LAB,POB 3354,RIYADH 11211,SAUDI ARABIA
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| Abstract | A newly developed and commercially available cellulose tris-4-methylphenyl benzoate (Chiralcel OJ) chiral stationary phase was used for the enantiomeric resolution of thalidomide enantiomers. A simple isocratic and direct liquid chromatographic resolution of racemic thalidomide was accomplished without any derivatization. Solvent system was hexane and ethanol (50:50) with the flow rate 1 ml/min. at 23-degrees-C. The capacity factor (k') for the first eluted enantiomer R-(+)-thalidomide was 9.67 and separation factor (alpha) obtained was 1.54. The maximum stereochemical resolution factor (R) obtained was 15.05. Since a large stereochemical resolution with baseline separation was achieved, this method could be used for a large scale preparative separation and also for optical purity determination of the drug in bulk and formulation dosage forms.
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ABOULENEIN HY, 1990, J CHROMATOGR SCI, V28, P307
BLASCHKE G, 1989, CHEM BER, V113, P2318
DE AU, 1975, J PHARM SCI, V64, P262
ERAVELLY J, 1977, LANCET, V1, P251
WAINER IW, 1987, J CHROMATOGR, V411, P139
WATERS MFR, 1974, LEPROSY REV, V45, P337
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