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Author(s) SASAKI K; KURATA K; FUNAYAMA K; NAGATA M; WATANABE E; OHTA S; HANAI N; NISHI T Title EXPRESSION CLONING OF A NOVEL ALPHA-1,3-FUCOSYL-TRANSFERASE THAT IS INVOLVED IN BIOSYNTHESIS OF THE SIALYL-LEWIS-X CARBOHYDRATE DETERMINANTS IN LEUKOCYTES Source JOURNAL OF BIOLOGICAL CHEMISTRY 269 (20): 14730-14737 Date 1994 MAY 20 Type Journal : Article LCR: 17   NCR: 55   LCS: 89   GCS: 269 Comment   Address KYOWA HAKKO KOGYO CO LTD,TOKYO RES LABS,MACHIDA,TOKYO 194,JAPAN. Abstract The sialyl Lewis x (NeuAc alpha 2-3Gal beta 1-4(Fuc alpha 1-3)Glc-NAc) determinants serve as ligands in the selectin-mediated adhesion of leukocytes to activated endothelium or platelets. In our efforts to identify glycosyltransferases involved in the biosynthesis of those ligands, we achieved expression cloning of a novel human alpha 1,3-fucosyltransferase termed Fuc-TVII from a THP-1 cDNA library by enrichment of the Namalwa cells highly expressing that determinant with a fluorescence-activated cell sorter. Expression of the COOH-terminal catalytic domain of Fuc-TVII showed an alpha 1,3-fucosyltransferase activity for a type II oligosaccharide with a terminal alpha 2,3-linked sialic acid among various accepters, consistent with that in vivo acceptor specificity. Alignment of the primary sequences of five alpha 1,3-fucosyltransferase and assignment of the chromosomal location of Fuc-TVII gene, together with that acceptor specificity, indicate that Fuc-TVII consists of a unique class of the alpha 1,3-fucosyltransferase family. Determination of the expression levels of these alpha 1,3-fucosyltransferases in various cells revealed that both Fuc-TVII and a myeloid fucosyltransferase Fuc-TIV were significantly expressed in myeloid Lineage cells. Fuc-TVII-transfected Namalwa cells exhibited significant binding to E-selectin in contrast to little binding of the Fuc-TIV-transfected cells. These results suggest that Fuc-TVII may participate in the biosynthesis of the selectin ligands. CR BERG EL, 1991, J BIOL CHEM, V266, P14869 BERG EL, 1992, BIOCHEM BIOPH RES CO, V184, P1048 BEVILACQUA MP, 1993, J CLIN INVEST, V91, P379 CHIRGWIN JM, 1979, BIOCHEMISTRY-US, V18, P5294 EASTON EW, 1993, BLOOD, V81, P2978 FEIZI T, 1985, NATURE, V314, P53 FOXALL C, 1992, J CELL BIOL, V117, P895 FUKUDA M, 1992, CELL SURFACE CARBOHY FUKUSHI Y, 1984, J BIOL CHEM, V259, P10511 FUKUSHIMA K, 1984, CANCER RES, V44, P5279 FURUYA A, 1992, ANTICANCER RES, V12, P27 GILLILAND G, 1990, P NATL ACAD SCI USA, V87, P2725 GOELZ SE, 1990, CELL, V63, P1349 GONWA TA, 1983, J IMMUNOL, V130, P706 HAKOMORI S, 1984, ANNU REV IMMUNOL, V2, P103 HAKOMORI S, 1991, CURR OPIN IMMUNOL, V3, P646 HANAI N, 1990, ANTICANCER RES, V10, P1579 HANDA K, 1991, BIOCHEM BIOPH RES CO, V181, P1223 HOLMES EH, 1993, ARCH BIOCHEM BIOPHYS, V301, P190 HOSOI S, 1991, CYTOTECHNOLOGY, V5, PS17 JOHNSON PH, 1987, BIOCHEM SOC T, V15, P396 KITAGAWA H, 1993, BIOCHEM BIOPH RES CO, V194, P375 KONDO A, 1990, AGR BIOL CHEM TOKYO, V54, P2169 KOSZDIN KL, 1992, BIOCHEM BIOPH RES CO, V187, P152 KOZAK M, 1989, J CELL BIOL, V108, P229 KUKOWSKALATALLO JF, 1990, GENE DEV, V4, P1288 KUMAR R, 1991, J BIOL CHEM, V266, P21777 LAM KS, 1991, NATURE, V354, P82 LARKIN M, 1992, J BIOL CHEM, V267, P13661 LASKY LA, 1992, SCIENCE, V258, P964 LEVINOVITZ A, 1993, J CELL BIOL, V121, P449 LOBB RR, 1991, J IMMUNOL, V147, P124 LOWE JB, 1990, CELL, V63, P475 LOWE JB, 1991, J BIOL CHEM, V266, P17467 MARGOLSKEE RF, 1988, MOL CELL BIOL, V8, P2837 MATSUSHITA Y, 1991, EXP CELL RES, V196, P20 MEIER W, 1993, BIOCHEM J 1, V294, P25 MIYAJI H, 1990, CYTOTECHNOLOGY, V3, P133 MIYAJI H, 1990, CYTOTECHNOLOGY, V4, P173 MOLLICONE R, 1990, EUR J BIOCHEM, V191, P169 MUROI K, 1992, BLOOD, V79, P713 NAKAMURA K, 1994, CANCER RES, V54, P1511 NORGARD KE, 1993, J BIOL CHEM, V268, P12764 PHILLIPS ML, 1990, SCIENCE, V250, P1130 POLLEY MJ, 1991, P NATL ACAD SCI USA, V88, P6224 SAKO D, 1993, CELL, V75, P1179 SANGER F, 1977, P NATL ACAD SCI USA, V74, P5463 SASAKI K, 1993, J BIOL CHEM, V268, P22782 SATO S, 1983, J TISSUE CULT METHOD, V8, P167 SHITARA K, 1987, CANCER RES, V47, P1267 TAKADA A, 1991, BIOCHEM BIOPH RES CO, V179, P713 VIEIRA J, 1987, METHOD ENZYMOL, V153, P3 WALZ G, 1990, SCIENCE, V250, P1132 WESTON BW, 1992, J BIOL CHEM, V267, P24575 WESTON BW, 1992, J BIOL CHEM, V267, P4152