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Author(s) Cate JH; Gooding AR; Podell E; Zhou KH; Golden BL; Kundrot CE; Cech TR; Doudna JA Title Crystal structure of a group I ribozyme domain: Principles of RNA packing Source SCIENCE 273 (5282): 1678-1685 Date 1996 SEP 20 Type Journal : Article LCR: 20   NCR: 70   LCS: 172   GCS: 728 Comment   Address UNIV COLORADO,DEPT CHEM & BIOCHEM,BOULDER,CO 80309. YALE UNIV,DEPT MOL BIOPHYS & BIOCHEM,NEW HAVEN,CT 06520. UNIV COLORADO,HOWARD HUGHES MED INST,BOULDER,CO 80309. Abstract Group I self-splicing introns catalyze their own excision from precursor RNAs by way of a two-step transesterification reaction. The catalytic core of these ribozymes is formed by two structural domains. The 2.8-angstrom crystal structure of one of these, the P4-P6 domain of the Tetrahymena thermophila intron, is described. In the 160-nucleolide domain, a sharp bend allows stacked helices of the conserved core to pack alongside helices of an adjacent region. Two specific long-range interactions clamp the two halves of the domain together. a two-Mg2+- coordinated adenosine-rich corkscrew plugs into the minor groove of a helix, and a GAAA hairpin loop binds to a conserved 11-nucleotide internal loop. Metal-and ribose-mediated backbone contacts further stabilize the close side-by-side helical packing, The structure indicates the extent of RNA packing required for the function of large ribozymes, the spliceosome, and the ribosome. CR 1994, ACTA CRYSTALLOGR D, V50, P760 BARTEL DP, 1991, CELL, V67, P529 BATTISTE JL, 1994, BIOCHEMISTRY-US, V33, P2741 BREAKER RR, 1994, CHEM BIOL, V1, P223 BREAKER RR, 1995, CHEM BIOL, V2, P655 BRUNGER A, 1993, XPLOR MANUAL VERSION BURKE JM, 1987, NUCLEIC ACIDS RES, V15, P7217 CARSON M, 1991, J APPL CRYSTALLOGR 5, V24, P958 CATE JH, 1996, SCIENCE, V273, P1696 CATE JH, IN PRESS STRUCTURE CATE JH, UNPUB CECH TR, 1990, ANNU REV BIOCHEM, V59, P543 CECH TR, 1993, GENE, V135, P33 CECH TR, 1994, NAT STRUCT BIOL, V1, P273 CELANDER DW, 1990, BIOCHEMISTRY-US, V29, P1355 CELANDER DW, 1991, SCIENCE, V251, P401 CHRISTIAN EL, 1993, BIOCHEMISTRY-US, V32, P4475 COLLINS RA, 1988, NUCLEIC ACIDS RES, V16, P2705 COSTA M, 1995, EMBO J, V14, P1276 CRICK FHC, 1968, J MOL BIOL, V38, P367 CUENOUD B, 1995, NATURE, V375, P611 DOUDNA JA, 1993, P NATL ACAD SCI USA, V90, P7829 DOUDNA JA, 1995, RNA, V1, P36 DOWNS WD, 1996, RNA, V2, P718 FLOR PJ, 1989, EMBO J, V8, P3391 GOLDEN BL, UNPUB GROSSHANS CA, 1989, BIOCHEMISTRY-US, V28, P6888 GUERRIERTAKADA C, 1986, BIOCHEMISTRY-US, V25, P1509 HEUS HA, 1991, SCIENCE, V253, P191 HOLBROOK SR, 1977, NUCLEIC ACIDS RES, V4, P2811 JACK A, 1977, J MOL BIOL, V111, P315 JONES TA, 1991, ACTA CRYSTALLOGR A, V47, P110 JOYCE GF, 1989, NUCLEIC ACIDS RES, V17, P7879 KALLENBACH NE, 1977, Q REV BIOPHYS, V10, P138 KIM SH, 1973, SCIENCE, V179, P285 LAGGERBAUER B, 1994, EMBO J, V13, P2669 LATHAM JA, 1989, SCIENCE, V245, P276 LAY PA, 1989, INORG CHEM, V28, P3001 MICHEL F, 1989, NATURE, V342, P391 MICHEL F, 1990, J MOL BIOL, V216, P585 MICHEL F, 1990, NATURE, V347, P578 MIICHEL F, 1983, EMBO J, V2, P33 MOHR G, 1994, NATURE, V370, P147 MORAS D, 1980, NATURE, V288, P669 MURPHY FL, 1993, BIOCHEMISTRY-US, V32, P5291 MURPHY FL, 1994, J MOL BIOL, V236, P49 MURPHY FL, 1994, SCIENCE, V265, P1709 OTWINOWSKI Z, 1991, ISOMORPHOUS REPLACEM, P80 PETERSON RD, 1994, BIOCHEMISTRY-US, V33, P5357 PICCIRILLI JA, 1993, NATURE, V361, P85 PLEY HW, 1994, NATURE, V372, P111 PYLE AM, 1992, NATURE, V358, P123 PYLE AM, 1993, SCIENCE, V261, P709 ROBERTUS JD, 1974, NATURE, V250, P546 SALDANHA R, 1993, FASEB J, V7, P15 SCHEVITZ RW, 1979, NATURE, V278, P188 SCHINDELIN H, 1995, J MOL BIOL, V249, P595 SCOTT WG, 1995, CELL, V81, P995 SHEN LX, 1995, FASEB J, V9, P1023 SMITH D, 1992, J BIOL CHEM, V267, P2429 SMITH D, 1993, BIOCHEMISTRY-US, V32, P5273 STROBEL SA, 1996, BIOCHEMISTRY-US, V35, P1201 VANDERHORST G, 1991, P NATL ACAD SCI USA, V88, P184 WANG JF, 1992, SCIENCE, V256, P526 WANG JF, 1993, SCIENCE, V260, P504 WANG YH, 1994, J MOL BIOL, V236, P64 WOESE CR, 1990, P NATL ACAD SCI USA, V87, P8467 WOO NH, 1980, NATURE, V286, P346 WYATT JR, 1993, RNA WORLD, P465 ZARRINKAR PP, 1994, SCIENCE, V265, P918