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Author(s) GOULD KL; MORENO S; OWEN DJ; SAZER S; NURSE P Title PHOSPHORYLATION AT THR167 IS REQUIRED FOR SCHIZOSACCHAROMYCES-POMBE P34CDC2 FUNCTION Source EMBO JOURNAL 10 (11): 3297-3309 Date 1991 NOV Type Journal : Article LCR: 4   NCR: 99   LCS: 12   GCS: 349 Comment   Address UNIV OXFORD,DEPT BIOCHEM,MICROBIOL UNIT,CELL CYCLE GRP,OXFORD OX1 3QU,ENGLAND. Abstract Eukaryotic cell cycle progression requires the periodic activation and inactivation of a protein-serine/threonine kinase which in fission yeast is encoded by the cdc2+ gene. The activity of this gene product, p34cdc2, is controlled by numerous interactions with other proteins and by its phosphorylation state. In fission yeast, p34cdc2 is phosphorylated on two sites, one of which has been identified as Tyr15. Dephosphorylation of Tyr15 regulates the initiation of mitosis. To understand more completely the regulation of p34cdc2 kinase activity, we have identified the second site of phosphorylation as Thr167, a residue conserved amongst all p34cdc2 homologues. By analysing the phenotypes of cells expressing various position 167 mutations and performing in vitro experiments, we establish that Thr167 phosphorylation is required for p34cdc2 kinase activity at mitosis and is involved in the association of p34cdc2 with cyclin B. Dephosphorylation of Thr167 might also play a role in the exit from mitosis. CR ANDERSON NG, 1990, NATURE, V343, P651 AXTON JM, 1990, CELL, V63, P33 BLOW JJ, 1990, CELL, V62, P855 BOOHER R, 1986, MOL CELL BIOL, V6, P3523 BOOHER R, 1987, EMBO J, V6, P3441 BOOHER R, 1988, EMBO J, V7, P2321 BOOHER R, 1989, CELL, V57, P1009 BOOHER RN, 1989, CELL, V58, P485 BOULTON TG, 1990, SCIENCE, V249, P4 BRIZUELA L, 1987, EMBO J, V6, P3507 CISEK LJ, 1989, NATURE, V339, P679 COOPER JA, 1983, METHOD ENZYMOL, V99, P387 CROSS FR, 1988, MOL CELL BIOL, V8, P4675 DASSO M, 1990, CELL, V61, P811 DEAN AM, 1990, SCIENCE, V249, P1044 DOONAN JH, 1989, CELL, V57, P987 DRAETTA G, 1987, CELL, V50, P319 DRAETTA G, 1988, CELL, V54, P17 DRAETTA G, 1989, CELL, V56, P829 DRAETTA G, 1990, TRENDS BIOCHEM SCI, V15, P378 DUCOMMUN B, 1990, BIOCHEM BIOPH RES CO, V167, P301 DUNPHY WG, 1988, CELL, V54, P423 DUNPHY WG, 1989, CELL, V58, P181 DURKACZ B, 1985, MOL GEN GENET, V201, P543 DURSO G, 1990, SCIENCE, V250, P786 FEATHERSTONE C, 1991, NATURE, V349, P808 FELIX MA, 1989, EMBO J, V8, P3059 FELIX MA, 1990, EMBO J, V9, P675 FLEIG UN, 1991, MOL GEN GENET, V226, P432 FREEMAN RS, 1991, BIOCHEMISTRY-US, V30, P2293 FURUKAWA Y, 1990, SCIENCE, V250, P805 GAUTIER J, 1988, CELL, V54, P433 GAUTIER J, 1989, NATURE, V339, P626 GAUTIER J, 1990, CELL, V60, P487 GAUTIER J, 1991, EMBO J, V10, P177 GHIARA JB, 1991, CELL, V65, P163 GORIS J, 1989, FEBS LETT, V245, P91 GOULD KL, 1989, NATURE, V342, P39 GOULD KL, 1990, SCIENCE, V250, P1573 GUTZ H, 1974, HDB GENETICS, V1, P395 HADWIGER JA, 1989, P NATL ACAD SCI USA, V86, P6255 HAGAN I, 1988, J CELL SCI, V91, P587 HANKS SK, 1991, CURR OPIN STRUC BIOL, V1, P369 HINDLEY J, 1984, GENE, V31, P129 HIRT H, 1991, P NATL ACAD SCI USA, V88, P1636 HURLEY JH, 1990, SCIENCE, V249, P1012 JESSUS C, 1990, FEBS LETT, V266, P4 JIMENEZ J, 1990, EMBO J, V9, P3565 KAMPS MP, 1989, ANAL BIOCHEM, V176, P22 KINOSHITA N, 1990, CELL, V63, P405 KREK W, 1989, EMBO J, V8, P3071 KREK W, 1991, EMBO J, V10, P305 KUMAGAI A, 1991, CELL, V64, P903 LABBE JC, 1988, NATURE, V335, P251 LABBE JC, 1989, CELL, V57, P253 LABBE JC, 1989, EMBO J, V8, P3053 LEE MG, 1988, NATURE, V333, P676 LEE TH, 1991, CELL, V64, P415 LEHNER CF, 1990, EMBO J, V9, P3573 LEVIN LR, 1990, MOL CELL BIOL, V10, P1066 LEWIN B, 1990, CELL, V61, P743 LOHKA MJ, 1989, J CELL SCI, V92, P131 LORINCZ AT, 1984, NATURE, V307, P183 LUNDGREN K, 1991, CELL, V64, P1111 MANIATIS T, 1982, MOL CLONING MAUNDRELL K, 1990, J BIOL CHEM, V265, P10857 MEIJER L, 1989, EMBO J, V8, P2275 MENDENHALL MD, 1987, CELL, V50, P927 MENDENHALL MD, 1988, P NATL ACAD SCI USA, V85, P4426 MORENO S, 1989, CELL, V58, P361 MORENO S, 1990, NATURE, V344, P549 MORENO S, 1991, METHOD ENZYMOL, V194, P795 MORLA AO, 1989, CELL, V58, P193 MURRAY AW, 1989, NATURE, V339, P280 MURRAY AW, 1989, SCIENCE, V246, P614 NASH R, 1988, EMBO J, V7, P4335 NORBURY C, 1991, EMBO J, V10, P3321 NURSE P, 1981, NATURE, V292, P558 NURSE P, 1990, NATURE, V344, P503 OHKURA H, 1989, CELL, V57, P997 PAYNE DM, 1991, EMBO J, V10, P885 PICARD A, 1989, J CELL BIOL, V109, P3347 PIGGOTT JR, 1982, NATURE, V298, P391 PINES J, 1989, CELL, V58, P833 PINES J, 1990, NEW BIOL, V2, P389 PONDAVEN P, 1990, GENE DEV, V4, P9 REED SI, 1990, P NATL ACAD SCI USA, V87, P5697 RICHARDSON HE, 1989, CELL, V59, P1127 RUSSELL P, 1986, CELL, V45, P145 RUSSELL P, 1987, CELL, V49, P559 SAZER S, 1990, J CELL SCI 3, V97, P509 SIMANIS V, 1986, CELL, V45, P21 SOLOMON MJ, 1990, CELL, V63, P1013 SPURR NK, 1990, J DNA SEQUENCING MAP, V1, P49 SURANA U, 1991, CELL, V65, P145 THNG JPH, 1990, CELL, V63, P313 THORSNESS PE, 1987, J BIOL CHEM, V262, P10422 WITTENBERG C, 1990, CELL, V62, P225 YAMASHITA K, 1990, EMBO J, V9, P4331