Record 10327   View: Standard Glossary  HistCite Guide
Author(s): Ragg H (Ragg, Hermann); Kumar A (Kumar, Abhishek); Koster K (Koester, Katharina); Bentele C (Bentele, Caterina); Wang YJ (Wang, Yunjie); Frese MA (Frese, Marc-Andre); Prib N (Prib, Natalie); Kruger O (Krueger, Olaf)
Title: Multiple gains of spliceosomal introns in a superfamily of vertebrate protease inhibitor genes
Source: BMC EVOLUTIONARY BIOLOGY 9: Art. No. 208
Date: 2009 AUG 22
Document Type: Journal : Article
DOI: 10.1186/1471-2148-9-208
Language: English
Comment:  
Address: Univ Bielefeld, Dept Biotechnol, Fac Technol, D-33501 Bielefeld, Germany.
Univ Bielefeld, Ctr Biotechnol, D-33501 Bielefeld, Germany.
Med Univ Vienna, Inst Med Chem, Ctr Physiol & Pathophysiol, A-1090 Vienna, Austria.
Reprint: Ragg, H, Univ Bielefeld, Dept Biotechnol, Fac Technol, D-33501
Bielefeld, Germany.
E-mail: hr@zellkult.techfak.uni-bielefeld.de
abhishek.abhishekkumar@gmail.com
kko@zellkult.techfak.uni-bielefeld.de
caterina.bentele@meduniwien.ac.at
Yunjie.Wang@gmx.net
marc-andre_frese@gmx.de
natalieprib@online.de
o.krueger@cellca.de
Author Keywords:  
KeyWords Plus: GENOME EVOLUTION; THROMBIN INHIBITOR; SECRETORY PATHWAY; CHICKEN GENOME; TELEOST FISH; SERPINS; EXPRESSION; ORIGIN; FUGU; ORGANIZATION
Abstract: Background: Intron gains reportedly are very rare during evolution of vertebrates, and the mechanisms underlying their creation are largely unknown. Previous investigations have shown that, during metazoan radiation, the exon-intron patterns of serpin superfamily genes were subject to massive changes, in contrast to many other genes. Results: Here we investigated intron dynamics in the serpin superfamily in lineages pre-and postdating the split of vertebrates. Multiple intron gains were detected in a group of ray-finned fishes, once the canonical groups of vertebrate serpins had been established. In two genes, cooccurrence of non-standard introns was observed, implying that intron gains in vertebrates may even happen concomitantly or in a rapidly consecutive manner. DNA breakage/repair processes associated with genome compaction are introduced as a novel factor potentially favoring intron gain, since all non-canonical introns were found in a lineage of ray-finned fishes that experienced genomic downsizing. Conclusion: Multiple intron acquisitions were identified in serpin genes of a lineage of ray-finned fishes, but not in any other vertebrates, suggesting that insertion rates for introns may be episodically increased. The co-occurrence of non-standard introns within the same gene discloses the possibility that introns may be gained simultaneously. The sequences flanking the intron insertion points correspond to the proto-splice site consensus sequence MAG up arrow N, previously proposed to serve as intron insertion site. The association of intron gains in the serpin superfamily with a group of fishes that underwent genome compaction may indicate that DNA breakage/repair processes might foster intron birth.
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