Author(s): Imura T; Yanagishita H; Ohira J; Sakai H; Abe M; Kitamoto D

Title: Thermodynamically stable vesicle formation from glycolipid biosurfactant sponge phase

Source: COLLOIDS AND SURFACES B-BIOINTERFACES 43 (2): 115-121

Date: 2005 JUN 25

Document Type: Journal : Article

DOI:  

Language: English

LCR: 3   CR: 35   LCS: 2   GCS: 4

Comment:  

Address: Natl Inst Adv Ind Sci & Technol, AIST, Res Inst Innovat Sustainable Chem, Tsukuba, Ibaraki 3058565, Japan.
Sci Univ Tokyo, Fac Sci & Technol, Noda, Chiba 2788510, Japan.

Reprint: Kitamoto, D, Natl Inst Adv Ind Sci & Technol, AIST, Res Inst Innovat
Sustainable Chem, Tsukuba Cent 5-2,Higashi 1-1, Tsukuba, Ibaraki
3058565, Japan.

E-mail: dai-kitamoto@aist.go.jp

Author Keywords:  

KeyWords Plus:  

Abstract: Thermodynamically stable vesicle (L-alpha 1) formation from glycolipid biosurfactant sponge phase (L-3) and its mechanism were investigated using a "natural" biocompatible mannosyl-erythritol lipid-A (MEL-A)/L-alpha-ditauroylphosphatidylcholine (DLPC) mixture by varying the composition. The trapping efficiency for calcein and turbidity measurements clearly indicated the existence of three regions: while the trapping efficiencies of the mixed MEL-A/DLPC assemblies at the compositions With X-DLPC <= 0.1 or X-DLPC >= 0.8 were almost zero, the mixed assemblies at the compositions with 0.1 < X-DLPC < 0.8 had some trapping efficiency, and in particular the composition with X-DLPC = 0.3 exhibited the maximum trapping efficiency. Confocal laser scanning microscopy (CLSM) and freeze-fracture electron microscopy (FFEM) determined that the assemblies in the compositions with X-DLPC <= 0.1 were droplets with a sponge phase (L-3) with diameter from 2 to 20 mu m and those of X-DLPC >= 0.8 were multilamellar vesicles (L-alpha) with diameter from 2 to 10 mu m. Meanwhile, dynamic light scattering (DLS) measurement revealed that the average size of the vesicles at the composition of X-DLPC = 0.3 was 633.2 nm, which is remarkably small compared to other compositions. Moreover, the mixed vesicle solution at the composition of X-DLPC = 0.3 was slightly bluish and turbid and kept its dispersion stability at 25 degrees C for more than 3 months, indicating the formation of a thermodynamically stable vesicle (L-alpha 1). These results exhibited the formation of a thermodynamically stable vesicle (L-alpha 1) with a high dispersibility from the MEL-A/DLPC mixture. The asymmetric distribution of MEL-A and DLPC in the two vesicle monolayers caused by the difference in geometrical structures is very likely to have changed their self-assembled structure from a sponge phase (L-3) to a thermodynamically stable vesicle (L-alpha 1). (c) 2005 Elsevier B.V All rights reserved.