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Author(s): Xie XS; Yu J; Yang WY
Title: Perspective - Living cells as test tubes
Source: SCIENCE 312 (5771): 228-230
Date: 2006 APR 14
Document Type: Journal : Editorial Material
DOI:
Language: English
Comment:
Address: Harvard Univ, Dept Chem & Biol Chem, Cambridge, MA 02138 USA.
Reprint: Xie, XS, Harvard Univ, Dept Chem & Biol Chem, Cambridge, MA 02138 USA.
E-mail: xie@chemistry.harvard.edu
Author Keywords:
KeyWords Plus: STOKES-RAMAN SCATTERING; SINGLE-MOLECULE; GENE-EXPRESSION; RNA-
POLYMERASE; IN-VIVO; KINESIN; MICROSCOPY; LOCALIZATION; TRANSPORT;
MOVEMENT
Abstract: The combination of specific probes and advanced optical microscopy now allows quantitative probing of biochemical reactions in living cells. On selected systems, one can detect and track a particular protein with single-molecule sensitivity, nanometer spatial precision, and millisecond time resolution. Metabolites, usually difficult to detect, can be imaged and monitored in living cells with coherent anti-Stokes Raman scattering microscopy. Here, we describe the application of these techniques in studying gene expression, active transport, and lipid metabolism.
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