Author(s): Zhang WP; Zhang ZH; Wei K; Shen FY; Hou R; Zhang L; Yue BS

Title: Isolation and characterization of polymorphic tri-and tetra-nucleotide microsatellite loci for the south China tiger Panthera tigris amoyensis

Source: JOURNAL OF NATURAL HISTORY 40 (38-40): 2259-2263

Date: 2006 

Document Type: Journal : Article

Language: English

LCR: 2   CR: 12   LCS: 0   GCS: 0

Comment:  

Address: Sichuan Univ, Key Lab Bioresources & Ecoenvironm, Minist Educ, Coll Life Sci, Chengdu 610064, Sichuan, Peoples R China.
Honghe Univ, Dept Biol, Mengzi, Yunnan, Peoples R China.
Chengdu Res Base Giant Panda Breeding, Key Lab Reprod & Conservat Genet Endangered Wildl, Chengdu, Sichuan, Peoples R China.

Reprint: Yue, BS, Sichuan Univ, Key Lab Bioresources & Ecoenvironm, Minist Educ,
Coll Life Sci, Chengdu 610064, Sichuan, Peoples R China.

E-mail: bsyue@yahoo.com

Abstract: Eleven tri-and tetra-nucleotide microsatellite loci from the south China tiger, Panthera tigris amoyensis, were isolated, and characterized in this paper. Among these microsatellite loci, one locus was monomorphic, among the remaining 10 loci, the number of observed alleles for each locus in 57 individual tigers ranged from four to nine, the expected and observed heterozygosity ranged from 0.366 to 0.805 and 0.400 to 0.837, respectively, and the mean polymorphic information content (PIC) was 0.609. Moreover, two loci (1741 and FCA391) were compared between the domestic cat and the south China tiger and differences in their flanking regions were found (5.9% for F41 and 4.8% for FCA391). These 10 polymorphic tri-and tetra-nucleotide microsatellite markers would be very useful in the evaluation of tiger population structure and the genetic relationships among the individual tigers.